bit-MAP®

The one and only single-cell sequencing for microbes that’s 100x more efficient than conventional methods

bit-GEM

The world’s most diverse database – 1.3 billion sequences and growing – built on single-cell sequencing of microbes from natural samples

bit-QED

Custom bioinformatics and AI-driven enzyme engineering for dramatic improvements in accuracy at decreased time and cost

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bit-MAP® Sequence

The one and only single-cell sequencing technology for microbes that’s 100x more efficient than conventional methods

Single cell sequencing is the only method that can provide in depth functional and phylogenetic information.

Our patented technology uses microfluidics to capture and sequence individual bacterial genomes within isolated capsules. All biochemical reactions, including cell lysis and DNA amplification, take place within the capsule, eliminating conventional hurdles like isolation, culturing, and complex data processing. Our whole-genome reconstructions enable us to compare down to the sub-strain level, revealing not only genetic and functional information of individual bacteria, but also genetic differences between target and related strains.

Faster, cheaper, and vastly more accurate than typical microbe sequencing methods, bit-MAP powers massively parallel whole-genome sequencing and reduces the potential time for sequencing our planet’s microbe population from over 10,000 years to within a single generation.

Benefits

  • Quantitative sequencing and analysis of microbial genomes at single-cell resolution
  • Discovery of new species from unculturable and extremophile microbial samples
  • Capable of sequencing difficult samples, including low biomass and high diversity samples
  • Access to more detailed phylogenetic and functional information than 16S RNA
  • Reveal strain heterogeneity and identify prebiotic responders, pathogens, and more
  • Link mobile genetic elements and antibiotic resistance genes to their host microbes
  • Highly diverse and most complete sequences of any method
  • Longer contigs and gene clusters for more complete genomes than shotgun metagenomics
  • Visibility into biosynthetic gene clusters

By The Numbers

  • 100x more efficient gene analysis, including microbial dark matter, than shotgun metagenomics
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bit-GEM Catalog

The world’s most diverse database — 1.3 billion sequences and growing — built on single-cell sequencing of microbes from natural samples

Our bit-GEM database is the leading resource for searchable and highly usable microbe genome sequences.

Drawing on nature’s most potent microbial resources, including samples from environments like soil, marine water, hot springs, mining sites, and other extreme conditions, our database contains over a billion gene sequences that cannot be obtained by any other method—and perhaps most importantly, are not available in any public database.

Benefits

  • Extremophiles with potentially novel enzyme activities and functional requirements
  • Unique, free-to-operate, patentable sequences
  • Industry-wide applications, including healthcare, pharma, sustainability, food, materials, and chemicals

By The Numbers

  • 1.3 billion proprietary sequences currently in the database
  • 1 billion new sequences every year
  • ~60% from environmental samples
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bit-QED Evolve

Custom bioinformatics and AI-driven enzyme engineering for dramatic improvements in accuracy at decreased time and cost

Backed by a team of specialist bioinformaticians, chemoinformaticians, wet lab experts, and fermentation specialists, our unique bioinformatics platform finds, assays, and modifies enzymes from bitBiome’s vast library to meet your precise needs.

Our enzyme mining process uses both sequence-based target screening and 3D structure-based target enzyme screening to identify a short list of candidates in a matter of weeks. Directed evolution then uses chemoinformatic modeling combined with machine learning and large language models to identify the most relevant amino acid locations that influence enzyme activity, make necessary changes to optimize activity, and assay to ensure the changes had their intended effects. Our streamlined gene engineering process combines cycles of generating diverse mutants in silico followed by robotics-driven wet lab enzyme assays, getting smarter and more precise with each round.

Benefits

  • Reduces a 2-3 year process to months or even weeks
  • Skilled bioinformaticians and chemoinformaticians maximize conventional models and leverage proprietary ones
  • In silico and wet lab screening

By The Numbers

  • >500× faster 3D structure analysis than Google AlphaFold2
  • 24 hour bioinformatics results
  • 75% reduction in time to enzyme result
  • Up to 75% cost reduction compared to conventional methods

Talk to us about how we can help improve your processes, provide new candidates, and partner with you to power the future of biomanufacturing.